5FS9
Crystal structure of the G338E mutant of human apoptosis inducing factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-10-24 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.619, 114.930, 121.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 114.930 - 1.750 |
| R-factor | 0.17588 |
| Rwork | 0.175 |
| R-free | 0.19995 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fs8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.440 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.440 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.100 | 1.500 |
| Number of reflections | 128212 | |
| <I/σ(I)> | 8.3 | 0.9 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2 M LITHIUM SULFATE, 0.1M TRIS, PH 8.5, AND 25% POLY-ETHYLENE GLYCOL 3350 |
