5FR2
Farnesylated RhoA-GDP in complex with RhoGDI-alpha, lysine acetylated at K178
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 62 |
| Unit cell lengths | 176.979, 176.979, 63.896 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.245 - 3.350 |
| R-factor | 0.2374 |
| Rwork | 0.236 |
| R-free | 0.25770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1doa |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.786 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.240 | 3.620 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.520 | 1.310 |
| Number of reflections | 17709 | |
| <I/σ(I)> | 7.1 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.3 | 15.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M LITHIUMSULFATE 0.1 M BIS-TRIS PH5.5 25% PEG3350 30% DEXTROSE |
