5FR0
The details of glycolipid glycan hydrolysis by the structural analysis of a family 123 glycoside hydrolase from Clostridium perfringens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 99.530, 99.530, 95.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 95.310 - 1.750 |
R-factor | 0.18274 |
Rwork | 0.181 |
R-free | 0.22099 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fqe |
RMSD bond length | 0.016 |
RMSD bond angle | 1.640 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.700 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.110 | 0.480 |
Number of reflections | 55369 | |
<I/σ(I)> | 14.7 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.6 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |