5FQO
Crystal structure of M. musculus protein arginine methyltransferase PRMT6 with SAH and magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-17 |
Detector | DECTRIS PILATUS 3K |
Spacegroup name | I 41 |
Unit cell lengths | 78.620, 78.620, 118.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.702 - 1.899 |
R-factor | 0.1601 |
Rwork | 0.158 |
R-free | 0.19830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c08 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.131 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.500 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.040 | 0.170 |
Number of reflections | 27171 | |
<I/σ(I)> | 22.5 | 4.8 |
Completeness [%] | 96.4 | 84.4 |
Redundancy | 5 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 100 MM TRIS PH 8.0 200 MM MGCL2 10% PEG 6000 |