5FQG
The details of glycolipid glycan hydrolysis by the structural analysis of a family 123 glycoside hydrolase from Clostridium perfringens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 113.340, 113.340, 95.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 98.160 - 2.300 |
| R-factor | 0.22808 |
| Rwork | 0.226 |
| R-free | 0.27279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fqe |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.282 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.690 |
| Number of reflections | 32038 | |
| <I/σ(I)> | 16.7 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.2 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
