5FQE
The details of glycolipid glycan hydrolysis by the structural analysis of a family 123 glycoside hydrolase from Clostridium perfringens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.420, 115.030, 135.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.710 - 1.530 |
| R-factor | 0.15948 |
| Rwork | 0.158 |
| R-free | 0.18515 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.726 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.700 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.410 |
| Number of reflections | 209500 | |
| <I/σ(I)> | 16.2 | 2.6 |
| Completeness [%] | 98.8 | 99.4 |
| Redundancy | 4.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.20 M SODIUM FORMATE, 11% (W/V) POLYETHYLENE GLYCOL 3350, AND 0.1 M HEPES, PH 7 |
