5FPH
The GTPase domains of the immunity-related Irga6 dimerize in a parallel head-to-head fashion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 98.510, 98.510, 1289.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.978 - 3.200 |
| R-factor | 0.2977 |
| Rwork | 0.297 |
| R-free | 0.31710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tq2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.722 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (PHASER MR) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 214.900 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.170 | 0.770 |
| Number of reflections | 115430 | |
| <I/σ(I)> | 13.12 | 1.74 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.82 | 6.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 33% PEG 4000 3 % ISOPROPANOL 50 MM HEPES PH 7.0 |
