5FP9
Crystal structure of human KDM4D in complex with 3-aminopyridine-4- carboxylic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-08 |
| Detector | SATURN A200 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.416, 71.416, 150.292 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.500 - 2.000 |
| R-factor | 0.15897 |
| Rwork | 0.157 |
| R-free | 0.21141 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.035 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.030 |
| High resolution limit [Å] | 2.030 | 2.000 |
| Rmerge | 0.430 | 0.430 |
| Number of reflections | 25162 | |
| <I/σ(I)> | 16.1 | 4.4 |
| Completeness [%] | 92.5 | 94 |
| Redundancy | 6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 0.1M HEPES, PH 7.4, 2.2M AMSO4 |
