5FOD
Crystal structure of the P.falciparum cytosolic leucyl-tRNA synthetase editing domain (space group P1) containing deletions of insertions 1 and 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.600, 53.100, 62.700 |
| Unit cell angles | 88.40, 73.70, 89.80 |
Refinement procedure
| Resolution | 60.160 - 1.700 |
| R-factor | 0.20517 |
| Rwork | 0.203 |
| R-free | 0.25506 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PLASMODIUM KNOWLESI LEURS EDITING DOMAIN |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.607 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.900 |
| Number of reflections | 62051 | |
| <I/σ(I)> | 6 | 0.8 |
| Completeness [%] | 94.0 | 88 |
| Redundancy | 2.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES (PH 7.5), 20% (W/V) PEG 10000, AND 8% ETHYLENEGLYCOL |
