5FNA
Cryo-EM reconstruction of caspase-1 CARD
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TECNAI ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | DIRECT ELECTRON DE-16 (4k x 4k) |
| Number of Images | 200 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.8 |
| Software | IHRSR,SPIDER |
