5FMP
KstR, transcriptional repressor of cholesterol degradation in Mycobacterium tuberculosis, bound to the DNA operator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2015-10-23 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.530, 62.781, 98.647 |
Unit cell angles | 90.00, 89.90, 90.00 |
Refinement procedure
Resolution | 44.310 - 2.260 |
R-factor | 0.20539 |
Rwork | 0.202 |
R-free | 0.27039 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mnl |
RMSD bond length | 0.012 |
RMSD bond angle | 1.484 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.510 | 2.380 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.060 | 0.250 |
Number of reflections | 20253 | |
<I/σ(I)> | 8.9 | 1.7 |
Completeness [%] | 87.3 | 50.1 |
Redundancy | 2.4 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.1 | 0.2 M SODIUM SULFATE, 8% TACSIMATE, PH 5.1; 21.7% PEG 6000 |