5FML
Crystal structure of the endonuclease from the PA subunit of influenza B virus bound to the PB2 subunit NLS peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-04 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.380, 80.850, 37.910 |
| Unit cell angles | 90.00, 97.15, 90.00 |
Refinement procedure
| Resolution | 40.420 - 1.700 |
| R-factor | 0.22677 |
| Rwork | 0.224 |
| R-free | 0.27556 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wrt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.504 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.030 | 0.560 |
| Number of reflections | 22279 | |
| <I/σ(I)> | 12.68 | 1.84 |
| Completeness [%] | 98.2 | 93 |
| Redundancy | 2.72 | 2.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH 6.5, 25% PEG6000 |
