5FL7
Structure of the F1c10 complex from Yarrowia lipolytica ATP synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-05 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 169.500, 182.200, 193.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.366 - 3.500 |
R-factor | 0.2747 |
Rwork | 0.274 |
R-free | 0.30540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xok |
RMSD bond length | 0.004 |
RMSD bond angle | 0.448 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.190 | 3.600 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.180 | |
Number of reflections | 75882 | |
<I/σ(I)> | 9.84 | 0.61 |
Completeness [%] | 99.9 | 100 |
Redundancy | 19.47 | 19.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 100 MM TRIS-HCL, POLYETHYLENE GLYCOL 400, pH 8.0 |