5FKP
Crystal structure of the mouse CD1d in complex with the p99 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-12 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.170, 107.570, 110.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.190 - 1.800 |
R-factor | 0.20681 |
Rwork | 0.205 |
R-free | 0.23361 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g08 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.267 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.090 | 0.830 |
Number of reflections | 47272 | |
<I/σ(I)> | 11.1 | 2.4 |
Completeness [%] | 99.5 | 99.6 |
Redundancy | 6.1 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2M AMMONIUM TARTRATE AND 20% PEG4000 |