5FI1

Crystal Structure of the P-Rex1 DH/PH tandem in complex with Cdc42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	110
Detector technology	CCD
Collection date	2013-08-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9788
Spacegroup name	C 2 2 21
Unit cell lengths	67.310, 95.906, 277.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.122 - 3.203
R-factor	0.208
Rwork	0.205
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dfk 5d3w
RMSD bond length	0.003
RMSD bond angle	0.706
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.160	8.560	3.160
Rmerge	0.071	0.029	0.446
Total number of observations	43371
Number of reflections	12403
<I/σ(I)>	8.3
Completeness [%]	83.6	85.9	87.2
Redundancy	3.5	3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	294	Ammonium citrate, PEG 3350