5FGZ

E. coli PBP1b in complex with FPI-1465

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-15
Detector	RAYONIX MX-300
Wavelength(s)	1.00
Spacegroup name	P 2 21 21
Unit cell lengths	62.480, 63.220, 293.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.220 - 2.850
R-factor	0.2409
Rwork	0.238
R-free	0.29158
RMSD bond length	0.027
RMSD bond angle	1.710
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall
Low resolution limit [Å]	63.220
High resolution limit [Å]	2.850
Number of reflections	26383
<I/σ(I)>	23.4
Completeness [%]	95.1
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20% w/v PEG 3350, 0.2M potassium/sodium tartate, 0.1M Bis Tris pH 8.5