5FGW
Structure of Sda1 nuclease with bound zinc ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93.15 |
| Detector technology | CCD |
| Collection date | 2015-08-10 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.104, 66.538, 83.536 |
| Unit cell angles | 108.72, 90.01, 92.36 |
Refinement procedure
| Resolution | 33.132 - 1.950 |
| R-factor | 0.2161 |
| Rwork | 0.215 |
| R-free | 0.26010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.978 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.325 | |
| Number of reflections | 73933 | |
| <I/σ(I)> | 16.45 | 2.91 |
| Completeness [%] | 97.1 | 88.4 |
| Redundancy | 4.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 296 | 2.5uL of protein (26.7mg/mL) was mixed with 3.5uL of 90mM imidazole pH 8, 0.18M zinc acetate, 18% (w/v) PEG3000 |
