5FFW
Crystal structure of the bromodomain of human BRPF1 in complex with H4K5acK8ac histone peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.348, 93.177, 32.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.260 - 1.500 |
| R-factor | 0.21378 |
| Rwork | 0.212 |
| R-free | 0.24307 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lc2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.501 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.260 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.062 | 0.634 |
| Number of reflections | 43114 | |
| <I/σ(I)> | 21.5 | 3.2 |
| Completeness [%] | 99.4 | 96.2 |
| Redundancy | 10.8 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.2 M NaCl, 25% PEG3350, 0.1 M Hepes pH 7.5 |
