5FEB

Crystal structure of the Voltage-gated Sodium Channel Beta 2 subunit extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	28.700, 59.420, 71.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.636 - 1.350
R-factor	0.1738
Rwork	0.172
R-free	0.20610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fdy
RMSD bond length	0.009
RMSD bond angle	0.995
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.670	1.430
High resolution limit [Å]	1.170	1.350
Rmerge	0.071	1.933
Number of reflections	39170
<I/σ(I)>	20.36	1.81
Completeness [%]	99.4	96.2
Redundancy	14.08	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	PEG 6000