5FD3
Structure of Lin54 tesmin domain bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 75 |
| Detector technology | CCD |
| Collection date | 2015-06-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03318 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.320, 53.880, 67.120 |
| Unit cell angles | 98.40, 102.53, 106.57 |
Refinement procedure
| Resolution | 36.000 - 2.420 |
| R-factor | 0.1836 |
| Rwork | 0.181 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rkh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.880 | 2.550 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.060 | 0.405 |
| Number of reflections | 18229 | |
| <I/σ(I)> | 8.86 | 2.2 |
| Completeness [%] | 95.1 | 94.4 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 21% PEG 3350, 310 mM Magnesium Chloride Hexahydrate |
