5FCN
microtubule binding domain of human CEP135
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-19 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 78.850, 78.850, 95.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.420 - 1.800 |
R-factor | 0.2167 |
Rwork | 0.216 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gkw |
RMSD bond length | 0.002 |
RMSD bond angle | 0.373 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1965) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.420 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.042 | 0.421 |
Number of reflections | 14195 | |
<I/σ(I)> | 18.71 | 3.49 |
Completeness [%] | 99.0 | 99 |
Redundancy | 4.8 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | 25% PEG 1500, 0.1M SPG buffer |