4GKW

Crystal Structure of the Coiled-coil Domain of C. elegans SAS-6

Summary for 4GKW

• Entry DOI: 10.2210/pdb4gkw/pdb
• Descriptor: Spindle assembly abnormal protein 6 (1 entity in total)
• Functional Keywords: double helix, sas-5, centriole, structural protein
• Biological source: Caenorhabditis elegans (nematode)
• Cellular location: Cytoplasm: O62479
• Total number of polymer chains: 2
• Total formula weight: 38723.34

Authors

Qiao, R.,Dong, G. (deposition date: 2012-08-13, release date: 2012-11-21, Last modification date: 2024-02-28)

Primary citation

Qiao, R.,Cabral, G.,Lettman, M.M.,Dammermann, A.,Dong, G.
SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly.
Embo J., 31:4334-4347, 2012

PubMed Abstract: The centriole is a conserved microtubule-based organelle essential for both centrosome formation and cilium biogenesis. Five conserved proteins for centriole duplication have been identified. Two of them, SAS-5 and SAS-6, physically interact with each other and are codependent for their targeting to procentrioles. However, it remains unclear how these two proteins interact at the molecular level. Here, we demonstrate that the short SAS-5 C-terminal domain (residues 390-404) specifically binds to a narrow central region (residues 275-288) of the SAS-6 coiled coil. This was supported by the crystal structure of the SAS-6 coiled-coil domain (CCD), which, together with mutagenesis studies, indicated that the association is mediated by synergistic hydrophobic and electrostatic interactions. The crystal structure also shows a periodic charge pattern along the SAS-6 CCD, which gives rise to an anti-parallel tetramer. Overall, our findings establish the molecular basis of the specific interaction between SAS-5 and SAS-6, and suggest that both proteins individually adopt an oligomeric conformation that is disrupted upon the formation of the hetero-complex to facilitate the correct assembly of the nine-fold symmetric centriole.

PubMed: 23064147
DOI: 10.1038/emboj.2012.280

Experimental method

X-RAY DIFFRACTION (3.3 Å)