5FCI
Structure of the vacant uL3 W255C mutant 80S yeast ribosome
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.15873 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 435.450, 287.660, 303.760 |
Unit cell angles | 90.00, 98.92, 90.00 |
Refinement procedure
Resolution | 49.993 - 3.400 |
R-factor | 0.2353 |
Rwork | 0.234 |
R-free | 0.28450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v88 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.319 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.993 | 3.500 | |
High resolution limit [Å] | 3.400 | 40.000 | 3.400 |
Rmerge | 0.348 | 0.109 | 1.413 |
Rmeas | 0.382 | 0.124 | 1.669 |
Total number of observations | 4845359 | ||
Number of reflections | 1009263 | 313 | 83863 |
<I/σ(I)> | 5.32 | 8.34 | 1.02 |
Completeness [%] | 99.9 | 46 | 100 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | Tris-Acetate pH 7.0, KSCN, Mg-Acetate, Glycerol, Spermidine, PEG20000 |