5FBJ

Complex structure of JMJD5 and substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-09-03
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	49.539, 65.052, 77.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.975 - 2.420
R-factor	0.1925
Rwork	0.190
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qu1
RMSD bond length	0.004
RMSD bond angle	0.925
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.975
High resolution limit [Å]	2.400
Number of reflections	7775
<I/σ(I)>	2
Completeness [%]	77.2
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		281	PEG3350 HEPEPS-Na