5FAO
CTX-M-15 in complex with FPI-1465
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-14 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.890, 62.730, 175.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.280 - 3.010 |
R-factor | 0.22576 |
Rwork | 0.223 |
R-free | 0.27154 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hbt |
RMSD bond length | 0.010 |
RMSD bond angle | 1.430 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.280 |
High resolution limit [Å] | 3.000 |
Number of reflections | 10061 |
<I/σ(I)> | 7.8 |
Completeness [%] | 98.3 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M ammonium sulfate, 0.1M MES pH6.5, 30% PEG5K monomethyl ether |