5F8H
Enterovirus 71 Polymerase Elongation Complex (C1S1/2 Form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97892 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.344, 77.081, 149.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.957 - 2.450 |
R-factor | 0.2004 |
Rwork | 0.198 |
R-free | 0.24420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ol6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.130 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (phenix.refine: 1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.000 | 60.000 | 2.540 |
High resolution limit [Å] | 2.450 | 5.280 | 2.450 |
Rmerge | 0.062 | 0.028 | 0.509 |
Rmeas | 0.070 | 0.032 | 0.574 |
Rpim | 0.032 | 0.015 | 0.259 |
Total number of observations | 130908 | ||
Number of reflections | 27772 | ||
<I/σ(I)> | 11 | ||
Completeness [%] | 99.7 | 98.2 | 99.9 |
Redundancy | 4.7 | 4.4 | 4.8 |
CC(1/2) | 0.999 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.085M MES pH 6.5, 25.5%(w/v) PEG 5000 monomethyl ether, 15%(v/v) Glycerol |