5F7E
Crystal structure of germ-line precursor of 3BNC60 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.857, 74.857, 83.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.327 - 1.900 |
| R-factor | 0.1996 |
| Rwork | 0.199 |
| R-free | 0.21370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jdv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.363 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10pre_2135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.327 | 36.327 | 2.000 |
| High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
| Rmerge | 0.025 | 0.803 | |
| Rmeas | 0.099 | ||
| Rpim | 0.038 | 0.011 | 0.339 |
| Total number of observations | 243616 | 7949 | 34347 |
| Number of reflections | 37312 | ||
| <I/σ(I)> | 16.1 | 56.6 | 2.6 |
| Completeness [%] | 99.6 | 99.1 | 98.5 |
| Redundancy | 6.5 | 6.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.1 | 298 | 0.1 M Bicine pH 9.1, 10% PEG 1500 |
