5F5O
Crystal structure of Marburg virus nucleoprotein core domain bound to VP35 regulation peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 32 |
Unit cell lengths | 98.396, 98.396, 95.730 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.910 - 2.200 |
R-factor | 0.21722 |
Rwork | 0.214 |
R-free | 0.27733 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.847 |
Data reduction software | SCALEPACK |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 52682 |
<I/σ(I)> | 36.78 |
Completeness [%] | 100.0 |
Redundancy | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 200 mM Ammonium sulfate, 100 mM Tris pH 8.5, 18% (wt/vol) Polyethylene glycol 3350 |