5F5K
E.Coli GlpG Y205F mutant complexed with aldehyde inhibitor in DMPC/CHAPSO bicelle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-20 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.972 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.320, 96.120, 62.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.400 |
R-factor | 0.23707 |
Rwork | 0.234 |
R-free | 0.28807 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.348 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.010 |
High resolution limit [Å] | 2.300 |
Number of reflections | 9474 |
<I/σ(I)> | 5.8 |
Completeness [%] | 97.6 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.5M NaCl, 0.1M Sodium Acetate pH 5, 5% Glycerol, 7% DMPC/CHAPSO bicelle |