5F5H
X-ray structure of Roquin ROQ domain in complex with Ox40 hexa-loop RNA motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.25363 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 89.660, 115.790, 42.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.230 |
| R-factor | 0.2183 |
| Rwork | 0.216 |
| R-free | 0.25687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qi0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.068 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.059 | 0.683 |
| Number of reflections | 21018 | |
| <I/σ(I)> | 14.9 | 2.1 |
| Completeness [%] | 98.7 | 97.7 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | PEG 3350, NaCl, Bis-TRIS |
