5F5G
Structure of E.Coli GlpG Y205F mutant complexed with peptidic inhibitor Ac-RMA-CHO in the DMPC/CHAPSO bicelle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.981 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.830, 97.500, 62.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.300 |
| R-factor | 0.20911 |
| Rwork | 0.207 |
| R-free | 0.25476 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.485 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 10422 | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 91.3 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.5M NaCl, 0.1M sodium acetate pH 4.5, 5% Glycerol, 7% DMPC/CHAPSO bicelle |
