5F5B
Structure of E.Coli GlpG complexed with peptidic inhibitor Ac-VRMA-CHO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.972 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 110.590, 110.590, 126.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ic8 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.478 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.010 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 20167 |
| <I/σ(I)> | 8.2 |
| Completeness [%] | 99.3 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 4M NaNO3, 0.1M Tris pH7.5, 5% Glycerol, 0.2 % nonyl glucoside |
