5F4Z
The crystal structure of an epoxide hydrolase from Streptomyces carzinostaticus subsp. neocarzinostaticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.356, 93.068, 134.059 |
| Unit cell angles | 90.00, 115.33, 90.00 |
Refinement procedure
| Resolution | 33.267 - 1.820 |
| R-factor | 0.1619 |
| Rwork | 0.160 |
| R-free | 0.20040 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.850 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.062 | 0.661 |
| Number of reflections | 177945 | |
| <I/σ(I)> | 31 | 1.32 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.16M MgCl2, 0.08M Tris:HCl, 24% (w/v) PEG 4000, 20% (v/v) Glycerol |
