5F46

Crystal structure of an aminoglycoside acetyltransferase meta-AAC0020 from an uncultured soil metagenomic sample, apoenzyme form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-02-11
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	54.850, 61.807, 114.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.842 - 1.850
R-factor	0.2107
Rwork	0.209
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yfj
RMSD bond length	0.004
RMSD bond angle	0.781
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.034	0.405
Number of reflections	33193
<I/σ(I)>	0.577
Completeness [%]	97.2	97.1
Redundancy	6.9	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	4 M sodium formate