5F3B

Structure of myostatin in complex with chimeric RK35 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2010-08-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	66.090, 67.616, 78.559
Unit cell angles	78.98, 88.77, 67.44

Refinement procedure

Resolution	49.170 - 1.760
R-factor	0.1807
Rwork	0.179
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gcy
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.760
Number of reflections	104791
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Chimeric RK35 Fab and myostatin were purified and the protein complex was concentrated to 10.75 mg/ml in a buffer of 50 mM tris hydrochloride pH 7.5 and 100 mM sodium chloride. Crystals were obtained using the hanging drop method with equilibration at 18C against a solution containing 20% PEG MME 5000 and 100 mM bis-tris pH 6.5