5F1I
MHC with 9-mer peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-05-12 |
| Detector | BIODIFF |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 84.101, 94.626, 124.355 |
| Unit cell angles | 84.60, 72.79, 81.61 |
Refinement procedure
| Resolution | 41.545 - 2.904 |
| R-factor | 0.2101 |
| Rwork | 0.208 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xpg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.480 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.696 |
| Number of reflections | 97116 |
| <I/σ(I)> | 6.322 |
| Completeness [%] | 97.9 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2 M potassium thiocyanate, pH 7.0, 20% PEG 3350 (w/v), 30% 1,4-Dioxane (v/v) |
