5F17

Structure of EAV NSP11 K170A mutant at 3.19A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	249.184, 249.184, 226.899
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.200
R-factor	0.2162
Rwork	0.215
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eyi
RMSD bond length	0.004
RMSD bond angle	0.864
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Number of reflections	44774
<I/σ(I)>	23	1.7
Completeness [%]	99.7	100
Redundancy	7.6	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	PEG