5F0W
Crystal structure of human copper homeostatic proteins atox1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-06 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 62 |
| Unit cell lengths | 112.493, 112.493, 56.634 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.480 - 2.700 |
| R-factor | 0.26235 |
| Rwork | 0.261 |
| R-free | 0.28935 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.622 |
| Data reduction software | HKL-3000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 10336 | |
| <I/σ(I)> | 17.776 | 4.667 |
| Completeness [%] | 90.2 | 87.4 |
| Redundancy | 6.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M tri-sodium citrate, 20 % (W/V) PEG 3350 |
