5EZQ
Venezuelan Equine Encephalitis Virus (VEEV) Nonstructural protein 2 (nsP2) Cysteine Protease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2015-01-16 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.805, 63.720, 86.078 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.210 - 1.660 |
R-factor | 0.20271 |
Rwork | 0.202 |
R-free | 0.21088 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hwk |
RMSD bond length | 0.006 |
RMSD bond angle | 1.163 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.720 | 1.760 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.375 | |
Number of reflections | 40129 | |
<I/σ(I)> | 21.91 | 3.24 |
Completeness [%] | 99.6 | 97.7 |
Redundancy | 6.01 | 2.95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | JCSG+ Condition #15 (0.1 M Bicine pH 8.5, 20% PEG 6000) |