5EZ5
Crystal structure of active Rab11A (S20V) in complex with GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 295 |
Detector technology | CCD |
Collection date | 2013-06-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 |
Unit cell lengths | 74.111, 74.111, 149.444 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.340 - 2.400 |
R-factor | 0.24033 |
Rwork | 0.237 |
R-free | 0.30380 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.005 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 15662 |
<I/σ(I)> | 30.8 |
Completeness [%] | 100.0 |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Sodium chloride, Tris, Magnessium chloride |