5EY1
Crystal structure of CodY from Staphylococcus aureus with GTP and Ile
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.824, 75.992, 166.301 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.249 - 2.000 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.23010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2B0L and 2B18 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.165 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.040 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.086 | 0.341 |
Number of reflections | 41162 | |
<I/σ(I)> | 17.03 | 3.2 |
Completeness [%] | 98.4 | 92.4 |
Redundancy | 5.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 10% (v/v) 2-propanol, 0.1 M tri-sodium citrate (pH 5.0), 26% PEG 400 |