5EY0
Crystal structure of CodY from Staphylococcus aureus with GTP and Ile
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.894, 76.028, 158.023 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.941 - 1.600 |
R-factor | 0.1793 |
Rwork | 0.178 |
R-free | 0.20910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2B0L and 2B18 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.076 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.072 | 0.313 |
Number of reflections | 69602 | |
<I/σ(I)> | 23.72 | 2.8 |
Completeness [%] | 94.3 | 92.8 |
Redundancy | 3.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | ammonium acetate, sodium citrate dehydrate, PEG400 |