5EWF
Ternary complex of human DNA polymerase eta inserting rCTP opposite an 8-Oxodeoxyguanosine Lesion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-31 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 61 |
Unit cell lengths | 99.124, 99.124, 81.384 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.922 - 1.782 |
R-factor | 0.1812 |
Rwork | 0.179 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o3p |
RMSD bond length | 0.008 |
RMSD bond angle | 1.146 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.115 | 1.229 |
Number of reflections | 43104 | |
<I/σ(I)> | 18.5 | 1.9 |
Completeness [%] | 99.2 | 99.1 |
Redundancy | 8.8 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | NaMES (pH 6.0), polyethylene glycol monomethyl ether 2000, and calcium chloride |