5EV3

Structure III of Intact U2AF65 Recognizing the 3' Splice Site Signal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 21 21 21
Unit cell lengths	43.351, 62.196, 77.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.870 - 1.500
R-factor	0.155
Rwork	0.153
R-free	0.18600
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	1.052
Data reduction software	XDS
Data scaling software	Aimless (0.1.26)
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.710	1.520
High resolution limit [Å]	1.500	1.500
Rmerge	0.024	0.148
Number of reflections	33918
<I/σ(I)>	38	6.5
Completeness [%]	98.2	69.8
Redundancy	6.1	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1M Succinic Acid, 0.1M, 1% PEG MME 2000