5EV2
Structure II of Intact U2AF65 Recognizing the 3' Splice Site Signal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1042 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.469, 115.220, 59.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.570 - 1.860 |
| R-factor | 0.154 |
| Rwork | 0.151 |
| R-free | 0.18800 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.116 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.26) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.570 | 1.900 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.303 | |
| Number of reflections | 17870 | |
| <I/σ(I)> | 24.6 | 4.6 |
| Completeness [%] | 98.7 | 95.9 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 200 mM LiCl, 100 mM sodium citrate, 8% PEG 6000, 10% PEG 300, 10% Dioxane, Deoxy-BigCHAP |
