5EUH
Crystal structure of the c-di-GMP-bound GGDEF domain of P. fluorescens GcbC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9771 |
| Spacegroup name | P 62 |
| Unit cell lengths | 141.913, 141.913, 106.169 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.452 - 2.989 |
| R-factor | 0.2273 |
| Rwork | 0.226 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i5c |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.699 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 3.100 |
| High resolution limit [Å] | 2.989 | 3.000 |
| Rmerge | 0.689 | |
| Number of reflections | 24701 | |
| <I/σ(I)> | 12.92 | 3.07 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 10.7 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.0 M Ammonium Sulfate, 0.1 M HEPES (pH 7.0), and 0.5% w/v PEG8000 |
