5ERJ
X-ray structure of cisplatin-encapsulated horse spleen apoferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.065 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.899, 180.899, 180.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.440 - 1.450 |
| R-factor | 0.14666 |
| Rwork | 0.145 |
| R-free | 0.17251 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.553 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.440 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.073 | 0.569 |
| Number of reflections | 45325 | |
| <I/σ(I)> | 17.2 | 3.5 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 11.3 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.6-0.8 M (NH4)2SO4, 0.1 M Tris pH 7.4-7.7, 50-60 mM CdSO4 |
