5ERD
Crystal structure of human Desmoglein-2 ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.968, 114.476, 226.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.395 - 2.900 |
| R-factor | 0.2437 |
| Rwork | 0.241 |
| R-free | 0.28680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q2w |
| RMSD bond length | 0.044 |
| RMSD bond angle | 1.270 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1810) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.395 | 2.990 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 3.085 | |
| Number of reflections | 53161 | |
| <I/σ(I)> | 10.7 | 0.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 26% EDO_P8K (Molecular Dimensions), 0.1M Tris-Bicine pH8.5, 0.1M amino acids mix (Molecular Dimensions), 0.01M Calcium chloride |
