5EP9
Crystal structure of the non-heme alpha ketoglutarate dependent epimerase SnoN from nogalamycin biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.207, 120.557, 102.879 |
| Unit cell angles | 90.00, 92.54, 90.00 |
Refinement procedure
| Resolution | 47.800 - 2.130 |
| R-factor | 0.17725 |
| Rwork | 0.176 |
| R-free | 0.20312 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ensemble of 2opw 2a1x 2fct |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.959 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 2.130 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.077 | 0.995 |
| Number of reflections | 76968 | |
| <I/σ(I)> | 11.7 | 1.4 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, ammonium acetate, Bis-Tris, ammonium ferric citrate, alpha ketoglutarate, sodium chloride |
