5EOZ
Mutagenicity of 7-Benzyl guanine lesion and Replication by Human DNA Polymerase beta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-05-03 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.754, 80.015, 55.589 |
Unit cell angles | 90.00, 107.76, 90.00 |
Refinement procedure
Resolution | 19.818 - 2.088 |
R-factor | 0.1863 |
Rwork | 0.183 |
R-free | 0.23820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
RMSD bond length | 0.009 |
RMSD bond angle | 1.291 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.088 |
Rmerge | 0.098 |
Number of reflections | 25149 |
<I/σ(I)> | 18.1 |
Completeness [%] | 100.0 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 14% to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5) |